Photosynthesis, a procedure carried out merely by workss, consists of a chemical procedure which transforms CO2 into organic compounds which such as sugar ( glucose ) and O, for this procedure to happen chemical energy is needed, or in other words sunlight. Furthermore, this procedure is taken out in the chloroplast of the works cell, located on the workss leaves, photosynthesis can besides happen in some algae.
In this experiment I am traveling to compare the different colourss that bromotimol blue alterations to and see if it is related to the photosynthetic activity of the algae ( Elodea ) used during the experiment.
The ground of this experiment is to find if bromotimol blue contributes to find if an aquatic works is transporting out photosynthesis or non, due to the fact that bromotimol bluish alterations colourss depending on the pH that the substance have: if the substance is acidic ( pH scope 6.0 or below ) the bromotimol blue should be xanthous, if the substance is impersonal ( pH scope 6.1 – 7.9 ) it should be green, and if the substance is a base ( pH scope 8.0 or higher ) it should be bluish. Due to these alterations of colour depending on the pH of the substance, bromotimol blue should be helpful to find when an aquatic works is during the procedure of photosynthesis.
The expected consequences are that the bromotimol blue will be utile as it changes colourss depending of the pH that it is exposed to, because due to this characteristic it can find whether the substance have a high concentration of CO2 or non, this is because CO2 have a impersonal pH which would turn the bromotimol blue to green. If the bromotimol bluish alterations from green to blue while the aquatic works is submerged into it, it would intend that the aquatic works is transporting out photosynthesis as the aquatic works should treat the CO2, as portion of the procedure of photosynthesis, cut downing its sum and besides altering the colour of the bromotimol blue to blue ( its original colour ) as the CO2 concentration have been reduced.
2. Research inquiry
Does the arrested development of CO2 in a bromotimol bluish solution, change the colour attesting the photosynthetic activity performed by a works?
The alterations of colour of the bromotimol bluish solution will grounds when photosynthetic activity is carried out by an aquatic works, as bromotimol bluish solution alterations color depending on the pH of the substance.
The variables identified in this experiment were:
Dependant variable: the colour of the bromotimol bluish solution, as it depends on the photosynthetic activity of a works.
Independent variable: the photosynthetic activity, due to the fact that it is non affected by any of the variables during the experiment.
Distance of the light bulb in the experiment
Concentration of bromotimol blue ( 1 % )
Size of the works: Elodea ( 6,5cm ± 0,05cm )
5. Materials and Methods
The stuffs used for this experiment were:
Spectophotometer ( A ±0,0005A )
Light bulb ( 100 W )
Bromotimol bluish solution 1 %
1 Elodea works sample
25ml graduated cylinder ( ml ± 0,05ml ) ? ? ?
15ml trial tubing ( ml ± 0,05ml )
2 beakers of 25ml
2 spectrophotometer cuvettes
Chronometer ( s ±0,0005s )
Test tubing rack
The method used in this experiment was:
First, 20 beads of bromotimol blue were assorted with 10ml of H2O in a beaker of 50ml.
The prepared solution was passed into a clean beaker so a straw would be placed inside the solution to blow in it by 30 seconds.
The solution was passed into a spectrophotometer cuvette, the optical density was read at 430nm.
The prepared solution was placed into a little trial tubing, so a little portion of Elodea ( aprox 6.5cm ± 0,05cm ) was besides placed indoors. The light bulb was turned and kept a distance off ( aprox. 10cm ± 0,05cm ) from the trial tubing that contained the Elodea for 15 proceedingss.
The optical density of the solution with the Elodea was read at 430nm.
Process # 4 was repeated with the old solution that was read, but alternatively of 15 proceedingss merely 5 proceedingss were waited.
Finally, procedure # 6 was repeated once more ; with these consequences a great assortment of optical densities can be compared.
6. Datas assemblage
Been realized the process, this is the natural information gathered:
While a straw was used to blow step N & A ; deg ; 1, it could be appreciated that the colour of the bromotimol blue changed from blue to green.
The measure in which the Elodea was inside the substance with some unreal energy, small bubbles emerging from the Elodea could be seen.
The substance changed to a light blue colour when the Elodea works was submerged in it transporting out photosynthetic activity.
Table N & A ; deg ; 1
Optical density measured in different minutes
Minutess waited ( s ±0,0005s )
Absorbance ( A ±0,0005A )
N & A ; deg ; 1 ( no alterations )
N & A ; deg ; 2
N & A ; deg ; 3
N & A ; deg ; 4
N & A ; deg ; 5
In Table N & A ; deg ; 1 it can be seen the different figure of steps and how much clip it was waited between steps, in the first two steps clip wasn?t taken, due to the fact that at those steps the Elodea wasn?t submerged in the substance, the step of clip started when the Elodea was submerged in the substance.
Image N & A ; deg ; 1
Bromotimol bluish colour in different steps
N & A ; deg ; 2
Measure N & A ; deg ; 1 ( no alterations )
N & A ; deg ; 3, 4, 5
In Image N & A ; deg ; 1 it can be seen that Measure N & A ; deg ; 1, the 1 that wasn?t exposed to CO2, have a strong blue colour ( original colour ) . While Measure N & A ; deg ; 2 have a green colour, after it have been exposed to CO2. In instance of Measure N & A ; deg ; 3, 4 and 5, these steps were indicated in one of the cuvettes due to the fact that the colour light blue is present on those 3 steps, the lone difference between them was that some steps were more clear between than other, as the figure of steps increased the substance became more clear, because as the steps increased the more clip the Elodea was submerged in the substance, uncluttering it more and more due to photosynthesis.
7. Datas analysis
From Table N & A ; deg ; 1, it can be seen that the optical density of the first substance, in other words the original, is the lowest one from all of the optical densities found during the experiment. One ground for this to hold happened is because the first substance did n’t had contact with anything that would change its concentration while the other substances were affected when CO2 was added straight to them when a straw was used to blow the substance. The ground for step N & A ; deg ; 2 to hold such an elevated optical density in comparing with # 1 is because at the clip CO2 was added its concentration increased and because concentration and optical density are depend from each other, if the concentration of the substance increased, in this instance of C02, its optical density would increase excessively.
From step N & A ; deg ; 2 to mensurate N & A ; deg ; 5 it can be observed of how the optical density is invariably diminishing, at this phase an Elodea works have been put inside the trial tubing where the substance is for it to get down photosynthesis ; so the ground for the optical density to maintain decreasing means that the concentration is diminishing every bit good and the lone manner for the concentration to lessening is through the decrease of the concentration of C02 and the lone manner available now for making such a thing is through the photosynthesis the Elodea is transporting out from the substance N & A ; deg ; 3 to N & A ; deg ; 5. Besides, it can be seen from the tabular array that when more clip is waited the optical density reduces even more, this can be seen in step N & A ; deg ; 3 in which the optical density reduces 0,202 ± 0,0005 of optical density by waiting 15 proceedingss, while the step N & A ; deg ; 4, in which have been waited 5 proceedingss, merely reduced 0,25 ± 0,0005 of optical density ; from this information it can be inferred that by more clip the Elodea works carry outs photosynthesis there would be less optical density ; in other words while clip passes the optical density will maintain decreasing, doing them indirectly relative, more clip, less optical density.
This avowal can be summarized in a graphic:
Graphic N & A ; deg ; 1
In Graphic N & A ; deg ; 1 it is showed how absorbance supports diminishing but at one point it will halt which is at the original optical density because it would be impossible for the substance, that has been added concentration to hold less optical density than the substance who had ne’er been in contact with other type of molecules.
From the qualitative informations observed before the alteration of colour of the bromotimol blue to green is because the pH of the substance is now impersonal which can be explained as CO2, with a impersonal pH, was added to the substance. And the other qualitative informations the bubbles from the Elodea was a mark that photosynthesis was taking topographic point because most of sea weed workss produce O while making photosynthesis, and this O produced can be seen from the bubbles that appear from the Elodea.
The alteration of colour in bromotimol blue determines that the Elodea has performed photosynthesis ; because it has already been proved that the concentration of C02 keeps diminishing doing the optical density lessening as an consequence of it, and the lone manner available of diminishing the concentration of CO2 in the optical density could be merely through the photosynthesis of the Elodea, which could be seen when some bubbles started to look attesting that photosynthesis was happening ; all of this factors would do the green bromotimol blue to alter to its original colour.
During the procedure of this experiment a restriction was found, this restriction was that unreal visible radiation was used during the experiment, which may hold affected the experiment as it is non certain to state that photosynthesis would be faster or slower at sunshine, so it would hold been a more specific informations if sunshine was used, due to the fact that it is the chief beginning which workss use for photosynthesis ; it would hold made a consequence closer to world.
Some suggestions for this experiment are: to prove with different light strengths to compare which light strength does workss be given to make photosynthesis faster and so determine if it affects the consequences, this manner more information is gathered, doing the experiment more accurate, and to wait more proceedingss for the works to transport out photosynthesis, in that manner more informations can be obtained, being more likely for accurate consequences.