Plasma degrees of matrix metalloproteinase-1 ( MMP-1 ) , -2 ( MMP-2 ) , and -7 ( MMP-7 ) have been shown to be independently correlated with ulcerative inflammatory bowel disease ( UC ) , but their relationship with each other and to disease badness remains ill-defined. This survey aims to measure the relationship between MMP-1, -2, and -7 and the badness of UC. Venous blood samples were collected from 50 patients with UC and 30 normal topics. MMP-1, -2, and -7 degrees were determined by enzyme linked immunosorbent check ( ELISA ) . Expression of plasma degrees in patients with UC was significantly higher than controls ( P & lt ; 0.001 ) , and was positively correlated with disease badness ( p & lt ; 0.01 ) . Plasma MMP-1, -2, and -7 degrees reflect their look in patients with UC. Plasma MMP-1, -2, and -7 degrees demonstrate the potency to go biomarkers to clinically name UC, predict its badness, and steer farther therapy.
Keywords: Matrix metalloproteinases ; MMP-1 ; MMP-2 ; MMP-7 ; Ulcerative inflammatory bowel disease.
Ulcerative inflammatory bowel disease ( UC ) is a chronic nonspecific inflammatory disease of the colon with unknown etiology. No specific effectual intervention is available at present. Pathological lesions are normally limited to the mucosal and submucosal countries, while diarrhoea, pusmucus bloody stool, and abdominal hurting and common clinical symptoms.
More attending is being focused on this disease because its morbidity has increased in recent old ages in Turkey, where UC was one time an uncommon GI upset. Previous surveies on the pathogenesis of UC have shown that ulceration are closely related to the inordinate debasement of extracellular matrix ( ECM ) by matrix metalloproteinases ( MMPs ) , which are over-expressed and activated to do colonic mucosal hurt and redness ( 1 ) . MMPs are group of zinc-dependent peptidases that are produced and secreted by connective tissue cells, endothelial cells, mono-macrophages, and other cells.
Animal and clinical surveies have revealed the over-expression of assorted MMPs in the inflammatory countries of colonic mucous membrane in UC ; MMPs look tend to be peculiarly high. It is believed that increased of MMP is one of the mechanisms in the pathogenesis of UC ( 2-4 ) . MMP-1 degrees are thought to be more closely associated with UC than other MMPs ; surveies ( 2, 5, 6 ) have reported that their look in patients with UC is significantly higher than in normal control topics. But, the correlativity between plasma degrees of these proteins and their looks or with UC disease badness is non clear. In this survey, we examined MMP-1, -2, and -7 plasma degrees to analyze their relationship and associations with disease badness in patients with UC.
Materials and Methods.
Fifty patients ( male, n=25 ; female, n=25 ; average age, 45 old ages ; age scope, 28-70 old ages ) with UC diagnosed by clinical symptoms, endoscopy, and pathology findings were enrolled in the survey. Of the 50 patients, 8 patients had pan-colon lesions, 6 had semicolon lesions, 24 had recto-sigmoid lesions, and 12 rectal lesions. Patients were divided into groups based on the diagnostic standards of UC badness: 21 patients were classified as mild ( M group ) and 29 patients as moderate- to-severe ( MTS group ) . Meanwhile, 30 normal topics were recruited as normal controls ( male, n=13 ; female, n=17 ; average age, 40 old ages ; age scope, 25-61 old ages ) .
Blood samples were collected after whirling at 1000 ten g for 30 proceedingss, and so at 10000 tens g for 10 proceedingss at 2 A° C to 8 A° C. Samples were stored at -80 A° C for ELISA.
Enzyme linked immunosorbent check ( ELISA ) for plasma MMP-1, -2, and -7 degrees were performed utilizing an ELISA kit ( R & A ; D Systems, USA ) following the maker ‘s instructions.
All values were expressed as average A± SD. Analysiss of discrepancy ( ANOVA ) trial were used. Spearman correlativity analysis was used to find the relationship between plasma degrees. P & lt ; 0.05 was considered statistically important.
The ELISA survey ( Table 1 ) showed that the overall plasma degrees of MMP-1, -2, and -7 in UC patients were significantly higher than those of the control group ( P & lt ; 0.001 ) . Plasma MMP-1 and -2 degrees in the MTS group were significantly higher than those of the M group and significantly higher in the M group compared to the normal controls ( P & lt ; 0.001 ) . However, no difference was observed between plasma MMP-7 degrees of patients and groups ( MTS versus M group ) ( p & gt ; 0.05 ) .
Plasma MMP-1 and -2 degrees were correlated in patients with UC. ( MMP-1, P & lt ; 0.01, r=0.85 ; MMP-2, P & lt ; 0.01, r=0.73 )
In this survey, we determined MMP-1, -2, and -7 degrees in UC patients compared with normal controls. The plasma degrees of MMP-1, -2, and -7 were determined utilizing ELISA. The degrees of these proteins increased in the plasma of UC patients compared to normal controls, and the degrees were correlated with disease badness ( except MMP-7 degrees ) .
Our consequences are consistent with findings from McKaig et Al and Wang et Al ( 4, 7 ) and supported old surveies showing that MMP-1 and MMP-2 reflected acute tissue hurt and was associated with the initial stairss of ulceration in UC and new blood vas formation ( 2, 4 ) .
MMPs degrees increased in the peripheral blood samples from patients with acute coronary syndrome, malignant neoplastic disease, hepatitis, and rheumatoid arthritis ( 8-12 ) . Wiercinska-Drapalo A et Al ( 13 ) used ELISA to find serum MMP-1 and found that serum MMP-1 was significantly increased in patients with UC compared with normal controls. Our survey produced similar findings ; plasma MMP-1, -2, and -7 degrees were higher in UC patients, but did non correlate with disease badness. These findings indicate that plasma MMP-1 and -2 by and large reflect the disease province. MMP-1 and -2 are chiefly associated with the initial stairss of ulceration in UC ( 2, 4 ) which may explicate its deficiency of association with disease badness. In our survey, most of the patients were enduring from chronic recurrent UC, so plasma MMP-1 and -2 could hold been affected.
Harmonizing to a old survey, ( 14 ) free MMP-1 and -2 decreased as the progressed. This may be another ground why plasma MMP-1 and -2 degrees increased merely in moderate-to-severe disease patients, but non mild patients.
Plasma MMP-1, -2, and -7 reflect their degrees to some extent ; particularly MMP-1 and -2. After excepting other diseases such as coronary bosom disease and liver disease that are besides associated with increased MMP-1 and MMP-2 degrees could perchance go utile biomarkers for UC diagnosing and disease badness, and may be utile to steer therapy.