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Dna Gel Electrophorosis Essay, Research Paper

Introduction:

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Deoxyribonucleic acid, Deoxyribonucleic acid, is a dual stranded, coiling nucleic

acid molecule which determines familial construction of a protein. The

? stairss? are made of bases: A, G, C, and T. The

sides are sugar and phosphate molecules. Restriction enzymes are

enzymes that cut DNA at limitation sites, go forthing fragments blunt or

sticky. The limitation fragments are separated utilizing a technique called

gel cataphoresis.

Deoxyribonucleic acid has a negative charge so when an electrical charge is

applied it makes DNA move to the positive side. Deoxyribonucleic acid is placed in

agarose gel. Smaller fragments move faster. The intent of this lab is to

separate Deoxyribonucleic acid fragments utilizing gel cataphoresis. Hind III cuts AAGCTT

between the two irst A? s. EcoRI cuts at GAATTC between the G and the

A. Hind III and EcoRI both make gluey terminals.

Consequences:

Our consequences for this lab were EcoRI separated into five fragments.

Hind III separated into four fragments. The control merely had one fragment.

( See chart A and figure 1-1 for distances )

Discussion:

The intent of this lab was to see how gel cataphoresis

offprints DNA fragments. We used Hind III, EcoRI, and a controlled

enzyme. Some fragments were difficult to see because of smearing. These

were the bigger fragments. Loading the Deoxyribonucleic acid was hard and if you

weren? T careful you could tear the Wellss which ruined the lab. We,

fortuitously, did non run into this job.

Abstraction:

The intent of this lab is to divide DNA fragments with gel

cataphoresis utilizing EcoRI and Hind III. Restriction enzymes are used to

interrupt up the Deoxyribonucleic acid, so negatively charged DN

A is placed in a gel

projecting tray. Then it is placed into an electrophoresis chamber. An

electrical field is placed across the agarose gel which forces the

fragments to travel down the gel. The sum of lines show how many

fragments there is in the Deoxyribonucleic acid. We had five fragments for EcoRI and six

for Hind III. The no enzyme had merely one fragment.

Procedures:

We sealed the terminals of a gel projecting tray with dissembling tape and

inserted a comb into the slots. The tray was filled about 6mm high with

agarose gel. It covered half the tallness of the comb. We waited 10s

proceedingss for the gel to solidify. Then we placed the tray in a gel box and

made sure that the comb was at a negative ( black ) terminal. The box was

filled with tris-borate-EDTA buffer so it covered the full surface of the

gel. The combs were removed without rending the Wellss. The micro pipet

was used to lade the lambda EcoRI, lambda Hind III, and lambda merely

into the Wellss. We dipped the pipet trough the surface of the buffer over

the Wellss and expelled the contents. The top of the cataphoresis

chamber was closed and electrical leads were connected. The dye was

observed as it moved shortly after the power supply was turned on. The

power supply was turned off after the sets migrated near the terminal of

the gel and the top of the cataphoresis chamber was removed. We

removed the gel from the gel projecting tray and examined it under a visible radiation

box and compared it to the ideal gel ( figure1-2 ) .

:

Restriction Enzymes: Cleavage of DNA lab

University of Illinois. ( 1999 ) . Experiment 2

Gel Electrophoresis of DNA. In

Molecular Biology Cyberlab, online:

Hypertext transfer protocol: //www.life.uluc.edu/molbio/geldigest/electro.html

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